Summary

The literature section presents previous knowledge about this protein. There have been five different efforts to annotate minimal organism genome. The annotations from those efforts are given in the Literature section for completeness.

AVX54711.1
JCVISYN3A_0302

Flavin reductase.
M. mycoides homolog: Q6MTP5.
TIGRfam Classification: 1=Unknown.
Category: Essential.

Statistics

Total GO Annotation: 40
Unique PROST Go: 6
Unique BLAST Go: 2
Unique Foldseek Go: 10

Total Homologs: 141
Unique PROST Homologs: 8
Unique BLAST Homologs: 0
Unique Foldseek Homologs: 32

Literature

Danchin and Fang [1]: water forming NADH oxidase|important for resolving the final step of fermentation
Yang and Tsui [2]: Na+ ABC transporter, ATP-binding component
Antczak et al. [3]: Oxygen-insensitive NAD(P)H nitroreductase
Zhang et al. [4]: GO:0046256|2,4,6-trinitrotoluene catabolic process
Bianchi et al. [5]: NADPH flavin oxidoreductase - (fre-like)

Structures and Sequence Alignment

The best structural homolog that predicted by 1. PBF was Q1CSR7 (Oxygen-insensitive NADPH nitroreductase) with a FATCAT P-Value: 0 and RMSD of 2.19 angstrom. The sequence alignment identity is 24.3%.
Structural alignment shown in left. Query protein AVX54711.1 colored as red in alignment, homolog Q1CSR7 colored as blue. Query protein AVX54711.1 is also shown in right top, homolog Q1CSR7 showed in right bottom. They are colored based on secondary structures.

  AVX54711.1 ---MQKEYIKELMLN-RKSARDFDLNKSISDQDLEIILTSMRMSPSAFNLMNLRLLIIDRNCSFKTELSP-LFYNQLNFI-NADKVILFVSDKTNKILNH 94
      Q1CSR7 MKFLDQEKRRRL-LNERHSCKMFDSHYEFSSTELEEIAEIARLSPSSYNTQPWHFVMV-TNKDLKKQIAAHSYFNE-EMIKSASALMVVCSLRPSELLPH 97

  AVX54711.1 TIDKTVNKMFNETQAEIANKFKKNVVSATSQL--AQIN----ELDNWSKTTAHITAG-IATIAAASL-NIDSCIIGGFN-AK---VLETFFIQKNYLSED 182
      Q1CSR7 --GHYMQNLYPES-------YKVRVIPSFAQMLGVRFNHSMQRLESYILEQCYIAVGQIC--MGVSLMGLDSCIIGGFDPLKVGEVLE-ERINK------ 179

  AVX54711.1 EQIVLTMSFGYMSKSI-----KPKPKIRIDENEYITFVK 216
      Q1CSR7 PKIACLIALG---KRVAEASQKSR-KSKVDA---ITWL- 210

Go Annotations

1. PBF indicates the go terms that are found by both PROST and BLAST and Foldseek.
2. PF indicates the go terms that are found by only PROST and Foldseek.
3. BF indicates the go terms that are found by only BLAST and Foldseek.
4. PB indicates the go terms that are found by both PROST and BLAST.
5. P indicates the go terms that are found by only PROST.
6. F indicates the go terms that are found by only Foldseek.
7. B indicates the go terms that are found by only BLAST.

Source GO Description
1. PBF GO:0018545 NAD(P)H nitroreductase activity
1. PBF GO:0016491 oxidoreductase activity
1. PBF GO:0008218 bioluminescence
1. PBF GO:0019439 aromatic compound catabolic process
1. PBF GO:0009636 response to toxic substance
1. PBF GO:0046256 2,4,6-trinitrotoluene catabolic process
2. PF GO:0016634 oxidoreductase activity, acting on the CH-CH group of donors, oxygen as acceptor
2. PF GO:0030659 cytoplasmic vesicle membrane
2. PF GO:2001039 negative regulation of cellular response to drug
2. PF GO:0140616 iodotyrosine deiodinase activity
2. PF GO:0006212 uracil catabolic process
2. PF GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
2. PF GO:0019740 nitrogen utilization
2. PF GO:0018546 nitrobenzene nitroreductase activity
2. PF GO:0009236 cobalamin biosynthetic process
2. PF GO:0006570 tyrosine metabolic process
2. PF GO:0010181 FMN binding
2. PF GO:0102919 5,6-dimethylbenzimidazole synthase activity
2. PF GO:0035527 3-hydroxypropionate dehydrogenase (NADP+) activity
2. PF GO:0000166 nucleotide binding
2. PF GO:0042403 thyroid hormone metabolic process
4. PB GO:0004155 6,7-dihydropteridine reductase activity
5. P GO:0004447 iodide peroxidase activity
5. P GO:0006208 pyrimidine nucleobase catabolic process
5. P GO:0005887 integral component of plasma membrane
5. P GO:0016657 oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor
5. P GO:0045922 negative regulation of fatty acid metabolic process
5. P GO:0034599 cellular response to oxidative stress
6. F GO:0052874 FMN reductase (NADH) activity
6. F GO:0052873 FMN reductase (NADPH) activity
6. F GO:0052619 coenzyme F420-1:gamma-L-glutamate ligase activity
6. F GO:0052618 coenzyme F420-0:L-glutamate ligase activity
6. F GO:0052645 F420-0 metabolic process
6. F GO:0008752 FMN reductase activity
6. F GO:0008152 metabolic process
6. F GO:0052890 oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
6. F GO:0008641 ubiquitin-like modifier activating enzyme activity
6. F GO:0005525 GTP binding
7. B GO:0005829 cytosol
7. B GO:0009635 response to herbicide

Uniprot GO Annotations

GO Description
GO:0016491 oxidoreductase activity

Homologs

1. PBF indicates the homologs that are found by both PROST and BLAST and Foldseek.
2. PF indicates the homologs that are found by only PROST and Foldseek.
3. BF indicates the homologs that are found by only BLAST and Foldseek.
4. PB indicates the homologs that are found by both PROST and BLAST.
5. P indicates the homologs that are found by only PROST.
6. F indicates the homologs that are found by only Foldseek.
7. B indicates the homologs that are found by only BLAST.

Source Homolog Description Fatcat Pvalue PROST Evalue BLAST Evalue Foldseek TMScore
1. PBF O25608 Oxygen-insensitive NADPH nitroreductase 0.00e+00 2.37e-57 6.27e-07 0.7996
1. PBF Q01234 Oxygen-insensitive NAD(P)H nitroreductase 0.00e+00 2.12e-44 1.92e-06 0.816
1. PBF P81102 Putative NAD(P)H nitroreductase YodC 0.00e+00 2.97e-51 2.57e-11 0.834
1. PBF Q1CSR7 Oxygen-insensitive NADPH nitroreductase 0.00e+00 2.83e-58 6.72e-07 0.8054
1. PBF Q4L920 Putative NAD(P)H nitroreductase SH0546 0.00e+00 2.13e-57 3.77e-10 0.8293
1. PBF Q99RB2 Putative NAD(P)H nitroreductase SAV2523 0.00e+00 6.10e-56 6.65e-10 0.8654
1. PBF Q5HD30 Putative NAD(P)H nitroreductase SACOL2534 0.00e+00 6.60e-56 6.85e-10 0.8635
1. PBF Q55233 Protein DrgA 0.00e+00 1.80e-38 1.05e-04 0.819
1. PBF Q4A087 Putative NAD(P)H nitroreductase SSP0379 0.00e+00 7.09e-58 2.68e-12 0.874
1. PBF Q9ZKP7 Oxygen-insensitive NADPH nitroreductase 0.00e+00 3.15e-59 4.11e-06 0.8037
1. PBF Q57431 Putative NAD(P)H nitroreductase 0.00e+00 4.96e-56 8.30e-16 0.8614
1. PBF Q5HLA1 Putative NAD(P)H nitroreductase SERP2086 0.00e+00 8.86e-55 3.11e-10 0.8628
1. PBF Q2YW87 Putative NAD(P)H nitroreductase SAB2397c 0.00e+00 6.10e-56 6.65e-10 0.8642
1. PBF Q17X32 Oxygen-insensitive NADPH nitroreductase 0.00e+00 7.93e-54 0.003 0.8443
1. PBF Q79ZZ6 Putative NAD(P)H nitroreductase MW2443 0.00e+00 6.10e-56 6.65e-10 0.8639
1. PBF Q6G6F2 Putative NAD(P)H nitroreductase SAS2409 0.00e+00 6.10e-56 6.65e-10 0.8622
1. PBF P96692 Putative NAD(P)H nitroreductase MhqN 0.00e+00 3.73e-56 0.043 0.7866
1. PBF Q8CN23 Putative NAD(P)H nitroreductase SE_2073 0.00e+00 3.52e-54 8.40e-10 0.8596
1. PBF O34475 Putative NAD(P)H nitroreductase YfkO 0.00e+00 1.18e-52 7.67e-14 0.8641
1. PBF Q2FDY2 Putative NAD(P)H nitroreductase SAUSA300_2462 0.00e+00 6.60e-56 6.85e-10 0.8615
1. PBF P46072 Major NAD(P)H-flavin oxidoreductase 0.00e+00 1.18e-46 1.64e-08 0.8086
1. PBF P96707 Putative NAD(P)H nitroreductase YdgI 0.00e+00 2.14e-50 1.27e-05 0.7513
1. PBF Q7A3H8 Putative NAD(P)H nitroreductase SA2311 0.00e+00 6.10e-56 6.65e-10 0.8617
2. PF B7LFB8 Probable malonic semialdehyde reductase RutE 5.55e-16 1.45e-38 NA 0.7496
2. PF O31571 Putative NAD(P)H nitroreductase YfhC 1.65e-06 2.76e-24 NA 0.6417
2. PF O28017 Putative NADH dehydrogenase/NAD(P)H nitroreductase AF_2267 4.49e-11 8.03e-18 NA 0.7606
2. PF B3Q6V6 Putative NADH dehydrogenase/NAD(P)H nitroreductase Rpal_4764 1.11e-15 5.53e-36 NA 0.7949
2. PF B7M8Z3 Probable malonic semialdehyde reductase RutE 6.66e-16 2.22e-35 NA 0.7749
2. PF B1W2E1 Putative NADH dehydrogenase/NAD(P)H nitroreductase SGR_2476 3.77e-15 4.11e-36 NA 0.7458
2. PF Q92PC8 5,6-dimethylbenzimidazole synthase 7.32e-11 9.19e-32 NA 0.6432
2. PF Q0TJ59 Probable malonic semialdehyde reductase RutE 5.55e-16 4.42e-35 NA 0.749
2. PF A7HZ77 Putative NADH dehydrogenase/NAD(P)H nitroreductase Plav_3612 5.00e-15 2.79e-35 NA 0.745
2. PF A1JMW8 Probable malonic semialdehyde reductase RutE 6.66e-16 2.29e-38 NA 0.7412
2. PF B1Y654 Putative NADH dehydrogenase/NAD(P)H nitroreductase Lcho_1290 2.56e-14 4.26e-43 NA 0.7763
2. PF B0RN85 Putative NADH dehydrogenase/NAD(P)H nitroreductase xcc-b100_0585 1.55e-15 4.96e-41 NA 0.7445
2. PF Q471I1 Putative NADH dehydrogenase/NAD(P)H nitroreductase Reut_A1586 5.00e-15 1.95e-43 NA 0.7325
2. PF B1X9C9 Probable malonic semialdehyde reductase RutE 7.77e-16 3.94e-38 NA 0.7744
2. PF A7MFX7 Probable malonic semialdehyde reductase RutE 4.44e-16 1.36e-42 NA 0.7506
2. PF Q5XW77 Chloronitrobenzene nitroreductase 2.78e-07 5.66e-29 NA 0.6073
2. PF Q32HQ3 Probable malonic semialdehyde reductase RutE 4.44e-16 4.50e-37 NA 0.75
2. PF Q3Z3A5 Probable malonic semialdehyde reductase RutE 4.44e-16 7.27e-36 NA 0.7868
2. PF Q60049 NADH dehydrogenase 0.00e+00 2.40e-56 NA 0.8539
2. PF Q5REW1 Iodotyrosine deiodinase 1 1.08e-10 1.66e-02 NA 0.691
2. PF B8GXF0 Putative NADH dehydrogenase/NAD(P)H nitroreductase CCNA_00059 6.77e-15 9.91e-37 NA 0.7836
2. PF Q3BY45 Putative NADH dehydrogenase/NAD(P)H nitroreductase XCV0587 3.33e-15 2.90e-41 NA 0.7438
2. PF Q4UZ74 Putative NADH dehydrogenase/NAD(P)H nitroreductase XC_0568 8.88e-16 4.96e-41 NA 0.7619
2. PF A4SXD4 Putative NADH dehydrogenase/NAD(P)H nitroreductase Pnuc_0932 9.99e-16 1.44e-41 NA 0.7973
2. PF B5YU50 Probable malonic semialdehyde reductase RutE 4.44e-16 3.71e-37 NA 0.8135
2. PF Q2RNG5 5,6-dimethylbenzimidazole synthase 2.87e-11 8.06e-38 NA 0.6348
2. PF P58792 Putative NADH dehydrogenase/NAD(P)H nitroreductase Atu2496 8.88e-16 3.55e-36 NA 0.7473
2. PF A7ZYW3 Probable malonic semialdehyde reductase RutE 6.66e-16 3.71e-37 NA 0.743
2. PF C4ZQD6 Probable malonic semialdehyde reductase RutE 5.55e-16 3.94e-38 NA 0.7861
2. PF A6T798 Probable malonic semialdehyde reductase RutE 6.66e-16 2.83e-41 NA 0.7382
2. PF Q8FJ44 Probable malonic semialdehyde reductase RutE 4.44e-16 1.02e-35 NA 0.7495
2. PF Q6DLR9 Nitrobenzene nitroreductase 3.33e-07 8.71e-27 NA 0.6064
2. PF B2SK49 Putative NADH dehydrogenase/NAD(P)H nitroreductase PXO_03909 3.55e-15 1.15e-41 NA 0.7444
2. PF O26223 Putative NADH dehydrogenase/NAD(P)H nitroreductase 2.29e-11 2.54e-23 NA 0.6978
2. PF B7UNZ1 Probable malonic semialdehyde reductase RutE 5.55e-16 3.26e-36 NA 0.8142
2. PF B6I984 Probable malonic semialdehyde reductase RutE 5.55e-16 1.45e-38 NA 0.7478
2. PF Q6TA49 Iodotyrosine deiodinase 1 1.02e-10 6.52e-03 NA 0.691
2. PF B1LIZ7 Probable malonic semialdehyde reductase RutE 5.55e-16 1.46e-35 NA 0.8136
2. PF A4W921 Probable malonic semialdehyde reductase RutE 6.66e-16 2.36e-39 NA 0.7556
2. PF Q83RV4 Probable malonic semialdehyde reductase RutE 5.55e-16 1.27e-37 NA 0.7758
2. PF O28082 Uncharacterized protein AF_2201 7.44e-07 4.11e-28 NA 0.518
2. PF D5AV14 5,6-dimethylbenzimidazole synthase 2.42e-11 8.95e-40 NA 0.6091
2. PF Q5GUV2 Putative NADH dehydrogenase/NAD(P)H nitroreductase XOO4267 3.66e-15 5.93e-41 NA 0.7556
2. PF A4VQH8 Putative NADH dehydrogenase/NAD(P)H nitroreductase PST_3601 7.77e-16 4.61e-40 NA 0.7951
2. PF B7NLB8 Probable malonic semialdehyde reductase RutE 5.55e-16 1.08e-36 NA 0.8138
2. PF B2U8S6 Putative NADH dehydrogenase/NAD(P)H nitroreductase Rpic_0870 3.11e-15 5.46e-42 NA 0.788
2. PF Q398D7 Putative NADH dehydrogenase/NAD(P)H nitroreductase Bcep18194_B1060 2.00e-15 5.34e-37 NA 0.7725
2. PF Q8XAU9 Probable malonic semialdehyde reductase RutE 4.44e-16 3.71e-37 NA 0.7496
2. PF A0R6D0 Nitroreductase NfnB 8.75e-07 1.92e-28 NA 0.5929
2. PF A1A9R3 Probable malonic semialdehyde reductase RutE 5.55e-16 3.11e-38 NA 0.7481
2. PF Q9AC08 Putative NADH dehydrogenase/NAD(P)H nitroreductase CC_0061 5.22e-15 9.91e-37 NA 0.7842
2. PF Q8GED9 Albonoursin synthase 4.12e-12 5.51e-25 NA 0.6784
2. PF P15888 Oxygen-insensitive NAD(P)H nitroreductase 0.00e+00 1.44e-41 NA 0.8146
2. PF Q8PPX9 Putative NADH dehydrogenase/NAD(P)H nitroreductase XAC0554 5.22e-15 2.42e-41 NA 0.7363
2. PF B2FKV6 Putative NADH dehydrogenase/NAD(P)H nitroreductase Smlt0482 3.66e-15 1.70e-40 NA 0.7501
2. PF P0CY55 5,6-dimethylbenzimidazole synthase 8.86e-11 2.51e-35 NA 0.6075
2. PF A7ZKB3 Probable malonic semialdehyde reductase RutE 5.55e-16 1.45e-38 NA 0.7484
2. PF P45244 Putative NAD(P)H nitroreductase HI_1542 3.94e-11 2.64e-20 NA 0.6769
2. PF Q8P4V1 Putative NADH dehydrogenase/NAD(P)H nitroreductase XCC3605 4.44e-15 4.96e-41 NA 0.7666
2. PF O30013 Putative NADH dehydrogenase/NAD(P)H nitroreductase AF_0226 5.55e-16 3.97e-22 NA 0.8253
2. PF Q9FBN0 Putative NADH dehydrogenase/NAD(P)H nitroreductase SCO5049 7.22e-15 1.84e-36 NA 0.7746
2. PF B5XXN4 Probable malonic semialdehyde reductase RutE 7.77e-16 1.93e-41 NA 0.7401
2. PF Q8Y0N9 Putative NADH dehydrogenase/NAD(P)H nitroreductase RSc1004 1.67e-15 1.89e-41 NA 0.788
2. PF B1IV89 Probable malonic semialdehyde reductase RutE 6.66e-16 3.71e-37 NA 0.7705
2. PF Q53009 Putative NADH dehydrogenase/NAD(P)H nitroreductase HadB 1.03e-13 4.35e-43 NA 0.7132
2. PF P0ACY2 Putative NAD(P)H nitroreductase YdjA 1.10e-10 2.20e-21 NA 0.6663
2. PF Q9FBV0 Putative NADH dehydrogenase/NAD(P)H nitroreductase SCO7141 2.02e-14 1.71e-42 NA 0.7551
2. PF Q2NY49 Putative NADH dehydrogenase/NAD(P)H nitroreductase XOO4023 3.55e-15 1.98e-41 NA 0.7393
2. PF Q1RDK9 Probable malonic semialdehyde reductase RutE 5.55e-16 3.11e-38 NA 0.8127
2. PF Q0T626 Probable malonic semialdehyde reductase RutE 4.44e-16 1.27e-37 NA 0.7497
2. PF B7MTF1 Probable malonic semialdehyde reductase RutE 5.55e-16 4.42e-35 NA 0.7876
2. PF B4SHM0 Putative NADH dehydrogenase/NAD(P)H nitroreductase Smal_0358 4.22e-15 4.34e-41 NA 0.7508
2. PF B7N3G4 Probable malonic semialdehyde reductase RutE 5.55e-16 1.08e-36 NA 0.758
2. PF B7MIF5 Probable malonic semialdehyde reductase RutE 5.55e-16 3.11e-38 NA 0.7753
2. PF Q5XCB9 Putative NAD(P)H nitroreductase Spy0809 0.00e+00 3.70e-54 NA 0.8324
2. PF B0T152 Putative NADH dehydrogenase/NAD(P)H nitroreductase Caul_0018 4.22e-15 1.25e-38 NA 0.7846
4. PB P38489 Oxygen-insensitive NAD(P)H nitroreductase 0.00e+00 2.42e-41 3.55e-04 NA
4. PB Q2FVA4 Putative NAD(P)H nitroreductase SAOUHSC_02829 0.00e+00 6.60e-56 6.85e-10 NA
5. P P75894 Probable malonic semialdehyde reductase RutE 7.77e-16 3.94e-38 NA NA
5. P Q9DCX8 Iodotyrosine deiodinase 1 1.50e-10 3.13e-03 NA NA
5. P P0ACY1 Putative NAD(P)H nitroreductase YdjA 1.32e-10 2.20e-21 NA NA
5. P Q6PHW0 Iodotyrosine deiodinase 1 1.05e-10 8.52e-03 NA NA
5. P Q5BK17 Iodotyrosine deiodinase 1 1.19e-10 1.71e-02 NA NA
5. P Q58779 Putative nitroreductase MJ1384 8.88e-12 2.05e-11 NA NA
5. P Q96VH4 Putative nitroreductase HBN1 1.30e-10 3.64e-25 NA NA
5. P P37261 Fatty acid repression mutant protein 2 2.49e-10 3.05e-19 NA NA
6. F Q7TWV3 Bifunctional F420 biosynthesis protein FbiB 1.16e-07 NA NA 0.5964
6. F P9WP78 Bifunctional F420 biosynthesis protein FbiB 1.15e-07 NA NA 0.5968
6. F Q6GC90 NADPH-dependent oxidoreductase 8.74e-13 NA NA 0.7035
6. F P23185 Microcin B17-processing protein McbC 1.00e-07 NA NA 0.5243
6. F Q56691 NADPH-flavin oxidoreductase 2.78e-13 NA NA 0.6753
6. F Q2FJN3 NADPH-dependent oxidoreductase 8.37e-13 NA NA 0.6963
6. F P9WM10 Uncharacterized protein MT1398 6.22e-02 NA NA 0.3871
6. F Q8CMQ4 NADPH-dependent oxidoreductase 9.87e-13 NA NA 0.7111
6. F Q75UN0 Bifunctional F420 biosynthesis protein FbiB 2.42e-08 NA NA 0.57
6. F Q9CCK2 Bifunctional F420 biosynthesis protein FbiB 1.18e-07 NA NA 0.5728
6. F Q49UU0 NADPH-dependent oxidoreductase 3.93e-13 NA NA 0.7065
6. F A5U7T6 Bifunctional F420 biosynthesis protein FbiB 1.11e-07 NA NA 0.5966
6. F A0QKC8 Bifunctional F420 biosynthesis protein FbiB 1.47e-07 NA NA 0.5827
6. F Q99WJ6 NADPH-dependent oxidoreductase 9.06e-13 NA NA 0.6961
6. F Q4L378 NADPH-dependent oxidoreductase 7.16e-13 NA NA 0.6852
6. F C1AH36 Bifunctional F420 biosynthesis protein FbiB 1.38e-07 NA NA 0.5964
6. F Q9KZK8 Bifunctional F420 biosynthesis protein FbiB 4.44e-08 NA NA 0.5808
6. F P39605 FMN reductase (NADPH) 2.35e-12 NA NA 0.6311
6. F Q5HRX9 NADPH-dependent oxidoreductase 9.32e-13 NA NA 0.7184
6. F P96977 CR(VI) reductase 2.95e-12 NA NA 0.6635
6. F Q2YVM4 NADPH-dependent oxidoreductase 8.44e-13 NA NA 0.6948
6. F Q8NY77 NADPH-dependent oxidoreductase 8.65e-13 NA NA 0.7036
6. F Q5YQS0 Bifunctional F420 biosynthesis protein FbiB 1.08e-07 NA NA 0.5929
6. F Q9Z5Z2 Oxygen-insensitive NADPH nitroreductase 1.20e-13 NA NA 0.6038
6. F Q5HIR4 NADPH-dependent oxidoreductase 8.26e-13 NA NA 0.7035
6. F Q6GJR6 NADPH-dependent oxidoreductase 8.62e-13 NA NA 0.6866
6. F A0QTG1 Bifunctional F420 biosynthesis protein FbiB 9.91e-08 NA NA 0.6148
6. F Q82DE3 Bifunctional F420 biosynthesis protein FbiB 4.57e-08 NA NA 0.6158
6. F P94424 FMN reductase [NAD(P)H] 2.57e-13 NA NA 0.6601
6. F A1KNR4 Bifunctional F420 biosynthesis protein FbiB 1.37e-07 NA NA 0.5961
6. F Q7A7J0 NADPH-dependent oxidoreductase 8.54e-13 NA NA 0.6962
6. F Q73UJ3 Bifunctional F420 biosynthesis protein FbiB 1.41e-07 NA NA 0.5937